Table T16. Marine medium salts used as a basis for cultivations.
| Component | Concentration |
|---|---|
| NaCl | 24.32 g/L |
| MgCl2·6H2O | 10 g/L |
| CaCl2·2H2O | 1.5 g/L |
| KCl | 0.66 g/L |
| Na2SO4 | 4.0 g/L |
| KBr | 0.84 mM |
| H2BO2 | 0.4 mM |
| SrCl2 | 0.15 mM |
| NH2PO4 | 0.4 mM |
| NaF | 0.07 mM |
| Na2SeO3 | 10–7 mM |
| NaWoO4 | 10–7 mM |
| Trace element solution | 1 mL/L |
| Autoclave, cool under anerobic conditions, and then add from sterile stock solutions: | |
| NaHCO3 (1M) | 30 mL |
| Vitamin solution | 2 mL |
| FeCl2 (1M in 0.1M HCl) | 0.5 mL |
| Na2S (1M) | 1.2 mL |
Note: Trace element solution is from Widdel et al., 1983. For oxic media, replace bicarbonate buffer with 2.38 g/L HEPES and cool under air atmosphere after autoclaving. HEPES = 4-(2-hydroxyethyl)-1-piperazinecthanesulfonic acid.
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