Figure F18. Subsampling equipment and procedure for 5 cm whole-round cores used for cell counting, cell cultivation, and FISH studies. A. Autoclaved stainless steel subcoring tool in scientist's hand used to (B) subcore the whole-round core, which is kept chilled (in autoclavable pan) by premolded gel ice packs. C. Small 2–3 cm3 sediment subsamples are placed into sterile plastic tubes (stored in an autoclavable plastic rack) and fixed with chilled sterile 2% paraformaldehyde in phosphate buffered saline ("PFA") solution. FISH samples are homogenized, incubated, washed, and stored for use (see above).
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