Table T11. Stable isotope–tracer incubation experiments for single cell–level metabolic assay, Expedition 329.
| Metabolism | Carbon source | Nitrogen source | Headspace of vial | ||||
|---|---|---|---|---|---|---|---|
| 13C substrate | Concentration | 15N substrate | Concentration | ||||
| (mM/vial) | (mM)* | (mM/vial) | (mM)* | ||||
| Autotrophy | Bicarbonate | 15 | 1.2 | Ammonia | 1.5 | 0.12 | 3.3% O2 in N2 |
| Heterotrophy | Glucose | 15 | 1.2 | Ammonia | 1.5 | 0.12 | 3.3% O2 in N2 |
| Heterotrophy | Pyruvate | 15 | 1.2 | Ammonia | 1.5 | 0.12 | 3.3% O2 in N2 |
| Heterotrophy | Acetate | 15 | 1.2 | Ammonia | 1.5 | 0.12 | 3.3% O2 in N2 |
| Heterotrophy (oligotrophy) | Amino acids† | 15 | 1.2 | Amino acids† | 15 | 1.2 | 3.3% O2 in N2 |
| Methanotrophy | Methane | ~0.7‡ | Ammonia | 1.5 | 0.12 | 1.7% O2, 43% N2, 43% 13CH4 (+15 psi) | |
| (Control without 13C) | No addition | 0 | 0 | Ammonia | 1.5 | 0.12 | 3.3% O2 in N2 |
| (Negative control 1) | No addition | 0 | 0 | No addition | 0 | 0 | 3.3% O2 in N2 |
| (Negative control 2: autoclaved sediment) | Bicarbonate glucose | 15 | 1.2 | Ammonia | 1.5 | 0.12 | 3.3% O2 in N2 |
* = tentative value based on 80% average porosity (accurate concentration of each sample was determined by actual porosity data measured onboard). † = mixture of 20 different kinds of 13C- and 15N-labeled amino acid (>99%). ‡ = as dissolved CH4 concentration. NanoSIMS and molecular ecological techniques were used.
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