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Table T4. Subsampling protocol for shipboard and shore-based samples collected during Expedition 334.

  O/H DOC DIC Halogens VFA Fe/Mo* REE B isotopes 7Li/6Li 87Sr/86Sr Noble gas isotopes Shipboard Total
Subsample
container: 		2 mL
screw top 		1.5 mL screw top 2 mL Agilent vials 2 mL
screw top 		Glass
vials 		Nalgene
8 mL bottle 		Nalgene
8 mL bottle 		4 mL HDPE bottle 4 mL Nalgene acidified to pH 2 2 mL Cu tubing 5 mL
tube 		4 mL HDPE bottle 4 mL HDPE bottle    
Treatment:   Frozen HgCl2 10 µL   Freeze at
–20°C 		HNO3 HNO3   HNO3 Room temperature   HNO3 ZnAc
>55 mL 2 1 2 2 3 10 8 2 3 2 6 4 7 52
50 mL 2 1 2 2 3 10 6 2 3 2 6 4 7 50
45 mL 2 1 2 2 2 8 5 2 3 2 6 4 6 45
40 mL 2 1 2 2 2 8   2 3 2 6 4 6 40
35 mL 2 1 2 2 2   5 2 3 2 6 4 4 35
30 mL 2 1 1 2 2 5   2 3 2 4 4 2 30
25 mL 2 1 1 2 2     2 3 2 4 4 2 25
20 mL 1 1 1 1 2       2 2 4 4 2 20
20 mL 1 1 1 1       2 2 2 4 4 2 20
15 mL 1     1   0 0 2   2 4 4 1 15
15 mL 1 1 1 1 2   0 0 3 0 1 3 2 15
10 mL 1   1         2 2 0 1 2 1 10
10 mL       1           2 4 2 1 10
5 mL 1     1             0.1 2 1 5.1
5 mL 1               1   0.1 2 1 5.1
2 mL 1                   0.1 1   2.1
1 mL 1                   0.1     1.1

* = Fe/Mo only to one core below sulfate–methane transition zone. Duplicate entries for a given volume (designated in bold) reflect alternate samples. DOC = dissolved organic carbon, DIC = dissolved inorganic carbon, VFA = volatile fatty acids, REE = rare-earth element. HDPE = high-density polyethylene.

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