Table T5. Sample log for microbiology sampling of the remotely operated vehicle (ROV) platform recovered from the old Hole 395A CORK.
| Sample ID | Description | Container | Used by/for |
|---|---|---|---|
| Area 1 | Rust around upper nut on ROV platform cone; light brown/orange | 50 mL conical | Mills/RNA (–80°C) |
| Area 2 | Base of platform arm; black to orange layer cake | 50 mL conical | Mills/RNA (–80°C) |
| Area 3 | Same as Area 2 | Whirl-pak bag | Edwards/DNA (–80°C) |
| Area 4 | Same as Area 2; underlying black rust | 50 mL conical | Mills/RNA (–80°C) |
| Area 5 | Base of 2nd arm; black to red-orange layer cake | 50 mL conical | Mills/RNA (–80°C) |
| Area 6 | Base of 3rd arm; similar to Area 3 | Whirl-Pak bag | Mills/RNA (–80°C) |
| Area 7A | ROV platform shackle; nicely scaled rusticle formations: some rusticles, some bulk, very wet | Whirl-Pak bag | Edwards/DNA (–80°C) |
| Area 7B | ROV platform shackle; nicely scaled rusticle formations: outside layer of rusticle | 50 mL conical | Mills/RNA (–80°C) |
| Area 7C | ROV platform shackle; nicely scaled rusticle formations: inside of rusticle | 50 mL conical | Mills/RNA (–80°C) |
| Area 7D | ROV platform shackle; nicely scaled rusticle formations: outside layer of rusticle | 50 mL conical | Mills/RNA (–80°C) |
| Area 7E | ROV platform shackle; nicely scaled rusticle formations: rusticle | 50 mL conical | Edwards/DNA (–80°C) |
| Area 8 | Top of Arm 3, black rust | 50 mL conical | Edwards/DNA (–80°C) |
| Area 9A | Base of Arm 4, black to orange layers; rusticle | 1.5 mL eppi | Edwards/FISH (–20°C) |
| Area 9B | Base of Arm 4, black to orange layers; rusticle | 1.5 mL eppi | Edwards/FISH (–20°C) |
| Area 9C | Base of Arm 4, black to orange layers; rusticle plus seawater | 1.5 mL eppi | Edwards/FISH (–20°C) |
| Area 9D | Base of Arm 4, black to orange layers; big chunk of rusticle | Whirl-Pak bag | Edwards/DNA (–80°C); Russell and Wang/culturing; archive (–80°C) |
| Area 10 | Base of cone, rusticle | 50 mL conical | Edwards/DNA (–80°C) |
| Area 11 | Base of cone, large chunk by nut, mostly black interior | Whirl-Pak bag | Mills/RNA (–80°C) |
| Area 12A–12E | Rust around nut on ROV platform cone | 1.5 mL eppis | Edwards/DNA (–80°C) |
| Area 13 | Second ROV platform shackle, rusticles | 2× 50 mL conical | Edwards/DNA (–80°C) |
| Area 14 | Rust around nut on ROV platform cone | 50 mL conical | Edwards/DNA (–80°C) |
| Area 15 | Base of Arm 5, rusticle | 50 mL conical | Edwards/DNA (–80°C) |
| Area 16 | Base of cone, rusticles | 2× 50 mL conical | Edwards/DNA (–80°C) |
Sample locations are illustrated in Figure F12. FISH = fluorescent in situ hybridization.
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