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Figure F1. qPCR analysis of sterilized North Pond sediments spiked with 106 cells/g of different species. Black = total cell counts for comparison, blue = cell numbers with extraction of DNA after Webster et al. (2003) without acid treatment, yellow = cell numbers with HI treatment, red = cell numbers with HCl treatment. 16S rRNA gene copy numbers per cell used for calculation of cell numbers: Escherichia coli = 5, Bacillus subtilis = 10, Methanohalobium evestigatum = 1.5.

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