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Table T10. Incubation for shore-based cultivation experiments, Expedition 337.

Target microbes Sample type Electron donor
(mM) 		Electron acceptor
(mM) 		Reducing
reagents 		BES
(40 mM) 		Incubation
temperature
(°C)
Methanogens
Hydrogenotrophic   Cuttings/in situ fluid H2 CO2 Na2S and cystein — 25–55
Cuttings/in situ fluid Formate (20) CO2 Na2S and cystein — 25–55
Aceticlastic   Cuttings/in situ fluid Acetate (20) CO2 Na2S and cystein — 25–55
Cuttings/in situ fluid Methanol (20) CO2 Na2S and cystein — 25–55
Homoacetogens   Cuttings/in situ fluid H2 CO2 Na2S and cystein + 25–55
Cuttings/in situ fluid Methanol (20) CO2 Na2S and cystein + 25–55
Iron reducers           Cuttings/in situ fluid Acetate (20) NTA-Fe(III) (10) — + 25–55
Cuttings/in situ fluid Acetate (10) Ferric citrate (30) — + 25–55
Cuttings/in situ fluid Acetate (20) Lepidocrocite (20) — + 25–55
Cuttings/in situ fluid Acetate (20) Goethite (20) — + 25–55
Cuttings/in situ fluid Acetate (20) Hematite (10) — + 25–55
Cuttings/in situ fluid Acetate (20) Magnetite (10) — + 25–55
Syntrophic oxidizers     Cuttings/in situ fluid Ethanol (20) Proton Na2S and cystein — 25–55
Cuttings/in situ fluid Propionate (20) Proton Na2S and cystein — 25–55
Cuttings/in situ fluid Butyrate (20) Proton Na2S and cystein — 25–55

BES = 2-bromoethane sulfonic acid, a methanogen-specific inhibitor, NTA = nitrilotriacetic acid. + = added, — = not added.

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