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Table T14. Stable isotope tracer incubation experiments for single-cell metabolic assay using NanoSIMS and molecular ecological techniques, Expedition 337.

No. 13C source 12C source 15N source D Headspace Target metabolic type Sampling depth
1 H13CO3–   15NH4+ D2O Ar Autotrophs 6 depths
2 H13CO3–   15N2 D2O Ar, 15N2* Autotrophs 6 depths
3 H13CO3–   15NH4+ D2O H2* Hydrogenotrophic autotrophs 6 depths
4 H13CO3– Glucose 15NH4+ D2O Ar Mixotrophs 6 depths
5 H13CO3– Formate 15NH4+ D2O Ar Mixotrophs 6 depths
6 H13CO3– Acetate 15NH4+ D2O Ar Mixotrophs 6 depths
7 H13CO3– Amino acids 15NH4+ D2O Ar Mixo-oligotrophs 6 depths
8 H13CO3– (Glucose, amino acids)   D2O Ar Autotrophs Max. 10 depths
9 H13CO3–       Ar Autotrophs 6 depths
10 13C18O   15NH4+ D2O Ar, 13C18O* Carboxydotrophs 6 depths
11 13C18O     D2O Ar, 13C18O* Carboxydotrophs 6 depths
12 13C18O Glucose 15NH4+ D2O Ar, 13C18O* Carboxydo-organotrophs 6 depths
13 13C18O Glucose   D2O Ar, 13C18O* Carboxydo-organotrophs 6 depths
14 13C18O       Ar, 13C18O* Carboxydotrophs 6 depths
15 13CH4   15NH4+ D2O Ar, 13CH4† Methanotrophs 6 depths
16 13CH4   15N2 D2O Ar, 13CH4†, 15N2† Methanotrophs 6 depths
17 13CH4       Ar, 13CH4† Methanotrophs 6 depths
18 H13CO3– CH4 15NH4+ D2O Ar, CH4* Methanotrophs 6 depths
19 H13CO3– CH4 15N2 D2O Ar, CH4*, 15N2† Methanotrophs 6 depths
20 13C-methylamine   14N-methylamine D2O H2* Hydrogenotrophic methylotrophs 6 depths
21 13C-methylamine   14N-methylamine D2O Ar Methylotrophs 6 depths
22 13C-methylamine   15NH4+ D2O Ar, H2* Hydrogenotrophic methylotrophs 6 depths
23 13C-methylamine     D2O Ar Methylotrophs 6 depths
24 13C-methanol   15NH4+ D2O Ar, H2* Hydrogenotrophic methylotrophs 6 depths
25 13C-methanol     D2O Ar Methylotrophs 6 depths
26 13C-glucose   15NH4+ D2O Ar Heterotrophs 6 depths
27 13C-glucose   15N2 D2O Ar, 15N2† Heterotrophs 6 depths
28 13C-glucose       Ar Heterotrophs 6 depths
29 13C-formate   15NH4+ D2O Ar Heterotrophs 6 depths
30 13C-formate   15N2 D2O Ar, 15N2† Heterotrophs 6 depths
31 13C-formate       Ar Heterotrophs 6 depths
32 13C-acetate   15NH4+ D2O Ar Heterotrophs 6 depths
33 13C-acetate   15N2 D2O Ar, 15N2† Heterotrophs 6 depths
34 13C-acetate       Ar Heterotrophs 6 depths
35 13C-benzoic acid (carboxyl)   15NH4+ D2O Ar Hydrocarbon degraders 1 from a lignite layer
36 13C-benzoic acid (ring, 13C6)   15NH4+ D2O Ar Hydrocarbon degraders 1 from a lignite layer
37 13C-toluene (methyl)   15NH4+   Ar Hydrocarbon degraders 1 from a lignite layer
38 13C-toluene (ring, 13C6)   15NH4+   Ar Hydrocarbon degraders 1 from a lignite layer
39 13C-benzene (ring, 13C6)   15NH4+   Ar Hydrocarbon degraders 1 from a lignite layer
40 13C-naphtalene (ring, 13C12)   15NH4+   Ar Hydrocarbon degraders 1 from a lignite layer
41 13C-ferulic acid (13C1,2,3)   15NH4+ D2O Ar Hydrocarbon degraders 1 from a lignite layer
42 13C-p-coumaric acid (13C1,2,3)   15NH4+ D2O Ar Hydrocarbon degraders 1 from a lignite layer
43 13C-vanillin (13C-methoxy and/or 13C-carbonyl)   15NH4+ D2O Ar Hydrocarbon degraders Max. 15 depths
44 13C-lignin (from maize)   15NH4+ D2O Ar Hydrocarbon degraders Max. 15 depths
45 13C-n-hexadecane (13C1,2)   15NH4+ D2O Ar Hydrocarbon degraders Max. 15 depths
46 13C,15N-amino acids   13C,15N-amino acids D2O Ar Oligotrophs 6 depths
47     15N-amino acids D2O Ar Oligotrophs 6 depths
48 13C-dNTP   15NH4+ D2O Ar Oligotrophs 6 depths
49     15NH4+ D2O Ar    
50     15N2 D2O Ar, 15N2†    
51       D2O Ar    
52       D218O Ar    

* = 5 mL of gas substrate was added, † = 5mL of 50% 13C or 15N gas substrate was added.

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