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Table T1. Results for most probable number enrichment cultures with different media incubated close to in situ temperature of 8°C under anaerobic conditions at different salinities, Expedition 347.

Core,
section
Depth
(mbsf)
Salinity
(‰)
Fe(OH)3
MnOOH
KNO3
KNO3 + Fe(II)
Polymer
Monomer
347-M0059C-
3H-2 8 25 10–2 10–2 10–4 10–2
5H-2 14 25 10–1 10–1 10–3 10–1
15H-1 45 20 10–1 10–1 10–1
21H-1 64 10
25H-1 78 10 10–1
347-M0060B-
13H-2 37 30 10–1 10–3 10–4 10–1
14H-2 41 30 10–1 10–3 10–1 10–4 10–1
23H-2 70 30 10–1 10–3 10–1 10–3 10–1
27H-2 82 30 10–1 10–1 10–3 10–1
28H-2 85 10 10–1 10–1 10–1 10–1
347-M0063E-
1H-2 2 1 10–3 10–3 10–3 10–4 10–1
6H-2 12 1 10–3 10–3 10–3 10–4 10–1
21H-2 41 5 10–2 10–1
27H-2 55 1 10–3 10–3 10–3 10–4 10–1
33H-2 66 5 10–1
40H-2 80 5 10–1
43H-2 90 5
347-M0065C-
4H-2 11 12 10–3 10–1 10–4 10–1 10–4 10–1
7H-2 20 12 10–3 10–1 10–3 10–1 10–4 10–1
10H-2 30 12 10–1 10–1
12H-2 37 1 10–1 10–1

Ferrihydrite, manganese oxide, or KNO3 were added as the electron acceptor each for enrichment of heterotrophs. Fe(II) was added for enrichment of nitrate-reducing lithotrophs. Mixtures of organic polymers or monomers served for enrichment of heterotrophic fermenters. Numbers = positive test tubes with the highest dilution step. — = no successful enrichment.

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