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Table T15. Culturing efforts, Hole U1376A.

Core, section,
interval (cm) 		Media Vials
inoculated
(N) 		Growth
330-U1376A-
2R-3, 0–6 Heterotrophs; 1% marine broth (low oxygen) 2 +
Heterotrophs; 10% marine broth (low oxygen) 2 ++
Heterotrophs; 1% marine broth (saturated oxygen) 2 ++
Heterotrophs; 10% marine broth (saturated oxygen) 2 ++
4R-1, 35–42 Heterotrophs; 1% marine broth (low oxygen) 2 –
Heterotrophs; 10% marine broth (low oxygen) 2 –
Heterotrophs; 1% marine broth (saturated oxygen) 2 Maybe
Heterotrophs; 10% marine broth (saturated oxygen) 2 ++
16R-5, 0–13 Autotrophic S oxidizers 2 –
Heterotrophic S oxidizers 2 Maybe
Autotrophic Fe reducers 2 ND
Heterotrophic Fe reducers 2 ND
Autotrophic Fe oxidizers 2 ND
Heterotrophs; 1% marine broth (low oxygen) 2 ++
Heterotrophs; 10% marine broth (low oxygen) 2 –
Heterotrophs; 1% marine broth (saturated oxygen) 2 Maybe
Heterotrophs; 10% marine broth (saturated oxygen) 2 –
17R-2, 112–120 Autotrophic S oxidizers 2 ++
Heterotrophic S oxidizers 2 ++
Autotrophic Fe reducers 2 ND
Heterotrophic Fe reducers 2 ND
Autotrophic Fe oxidizers 2 ND
Heterotrophs; 1% marine broth (low oxygen) 2 ++
Heterotrophs; 10% marine broth (low oxygen) 2 ++
Heterotrophs; 1% marine broth (saturated oxygen) 2 ++
Heterotrophs; 10% marine broth (saturated oxygen) 2 ++
Heterotrophic sulfur-oxidizing bacteria 2 ++
23R-1, 102–114 Autotrophic S oxidizers 2 Maybe
Heterotrophic S oxidizers 2 Maybe
Autotrophic Fe reducers 2 ND
Heterotrophic Fe reducers 2 ND
Autotrophic Fe oxidizers 2 ND
Heterotrophs; 1% marine broth (low oxygen) 2 Maybe
Heterotrophs; 10% marine broth (low oxygen) 2 ++
Heterotrophs; 1% marine broth (saturated oxygen) 2 Maybe
Heterotrophs; 10% marine broth (saturated oxygen) 2 Maybe

Analysis of growth was determined by shaking the vials. If turbidity was greater than in the initial sample, growth was considered positive. ++ = both vials were positive, + = one vial was positive, – = no growth. ND = not determined (media targeting iron reducers and iron oxidizers contain particulates, and therefore a change in turbidity was impossible to detect).

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