Table T4. Compilation of types and storage treatments for samples taken during microbiological sampling, Sites U1316, U1317, and U1318.
| Code | Sample type (cm) |
Sample | Storage treatment |
Sites sampled | |||
|---|---|---|---|---|---|---|---|
| WRC | Syringe | U1316 | U1317 | U1318 | |||
| HS | 5 | Methane headspace | Processed on ship | X | X | X | |
| CH4 | 5 | Detailed methane | Gas vial | X | X | X | |
| DEL | 5 | δ13C-methane | Gas vial | X | X | X | |
| HGA | 5 | Higher hydrocarbon analyses | Gas vial | X | X | X | |
| IW | 10–20 | Interstitial water | Processed on ship | X | X | X | |
| CONT-PFT | 5 | PFT contamination tests | Gas vial | X | X | X | |
| CONT-BEAD | 5 | Bead contamination tests | Gas vial | X | X | X | |
| AODC | 5 | Direct bacterial counts | Fomaldehyde | X | X | X | |
| AOM | 30 | Rates of anaerobic oxidation of methane | In nitrogen +4°C, anoxic | X | X | X | |
| SRR | 10 | Rates of sulfate reduction, dehalogenation experiments | In nitrogen +4°C, anoxic | X | X | X | |
| CH4C | 20 | Rates of methanogenesis and thymidine incorporation, MPN enrichments | In nitrogen +4°C, anoxic | X | X | X | |
| URI | 5 | Rates of hydrogenase activity | In nitrogen –80°C, anoxic | X | X | X | |
| GAS | 6 | Methane isotopes and higher hydrocarbon gas concentrations | +4°C poisoned (NaN3) | X | X | X | |
| VIR | 5 | Viren | In nitrogen –80°C, anoxic | X | X | X | |
| FISH | 5 | CARD-FISH | In nitrogen –80°C, anoxic | X | X | X | |
| DNAI | 5 | DNA-qPCR Div | –80°C | X | X | X | |
| DNAPCR | 5 | qPCR quantification of bacterial and archaeal populations | –80°C | X | X | X | |
| DNAS | 5 | DNA plasmid vectors | –80°C | X | X | ||
| DNAG | 5 | CARD-FISH and 16S rRNA analysis of AOM communities | –80°C | X | X | X | |
| DNAIODP | 5 | IODP DNA archive core | –80°C | X | X | X | |
| AALEO | 5 | Amino acids | –80°C | X | X | X | |
| DNAC | 10 | DNA extraction, amplification, DGGE, functional genes dsrAB and mcrA | –80°C | X | X | X | |
| LIPH | 5 | Intact phospholipids | –80°C | X | X | X | |
| WRCB | 30 | Oedometry | Room temperature | X | X | ||
| LIPD | 5 | Archaeal tetra-ether membrane lipids | –80°C | X | |||
| MICT | 5 | Microtomography | Room temperature | X | |||
Notes: PFT = perfluorocarbon tracer. MPN = most probable number, CARD-FISH = catalyzed reporter deposition–fluorescent in situ hybridization. IODP = Integrated Ocean Drilling Program. DNA = deoxyribonucleic acid. WRC = whole-round core. qPCR = quantitative polymerase chain reaction, rRNA = ribosomal ribonucleic acid, AOM = anaerobic oxidation of methane, DGGE = denaturing gradient gel electrophoresis, dsrAB = dissimilatory sulfite reductase, mcrA = methyl-coenzyme M reductase.
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