Table T8. Modified DSMZ medium 1040. (See table notes.)
| Component | Concentration per L |
Comments |
|---|---|---|
| Sigma sea salts | 35 g | |
| Yeast extract | 1 g | |
| Wolfe’s mineral elixir | 1 mL | DSMZ medium 792 |
| Resazurin | 0.5 mL | 1 g/L stock solution |
| Autoclave, then add the following: | ||
| 10% NaHCO3 | 20 mL | |
| Vitamin solution | 1 mL | 10 times concentrate of solution described in DSMZ medium 141; mixture of 10 vitamins |
| FeSO4·7H2O | 5 mL | 50 g/L stock solution |
| Na-dithionate | 1 mL | 25 g/L stock solution |
| Substrate | 10 mL | 1 M stock solution of acetate, lactate, pyruvate, or ethanol |
Notes: Adjust pH to 7.2 with H2SO4. Concentrations are per liter of Millipore 18.2 MΩ·cm Type 1 ultrapure water.
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