Table T8. Cultivation experiments using sediment and/or basalt samples, Expedition 329.
| Medium name | Solid/Liquid | Sample type | Target physiology | Electron donor | Electron acceptor | Carbon source | Nitrogen source | Incubation temperature (ºC) | pH | Remarks |
|---|---|---|---|---|---|---|---|---|---|---|
| SPG-1 | Liquid | Sediment | Aerobic hydrogenotrophic autotrophs | H2 | O2 | CO2 | NH4+ | 10 | 7.0 | |
| SPG-2 | Liquid | Sediment | Aerobic hydrogenotrophic autotrophs | H2, yeast extract | O2 | CO2, yeast extract | NH4+ | 10 | 7.0 | |
| SPG-3 | Liquid | Sediment | Hydrogenotrophic iron-reducing autotrophs | H2 | Amorphous FeOOH | CO2 | NH4+ | 10 | 7.0 | |
| SPG-4 | Liquid | Sediment | Heterotrophs | Glucose, peptone, yeast extract | O2 | Glucose, peptone, yeast extract | NH4+ | 10 | 7.0 | |
| SPG-5 | Liquid | Sediment | Nitrate-reducing, iron-oxidizing autotrophs | FeCl2 | NO3– | CO2 | NH4+ or NO3– | 10 | 7.0 | |
| SPG-6 | Liquid | Sediment | Hydrogenotrophic nitrate-reducing autotrophs | H2 | NO3– | CO2 | NH4+ or NO3– | 10 | 7.0 | |
| SPG-7 | Liquid | Sediment | Hydrogenotrophic nitrate-reducing autotrophs | H2, yeast extract | NO3– | CO2 | NH4+ or NO3– | 10 | 7.0 | |
| SPG-8 | Liquid | Sediment | Sulfur- and/or H2-oxidizing chemolithoautotrophs | H2, S2O32– | O2 | CO2 | NH4+ | 10 | 7.0 | |
| SPG-9 | Liquid | Sediment | Aerobic organotrophs | Tryptone, yeast extract | O2 | Tryptone, yeast extract | NH4+ | 10 | 7.0 | |
| SPG-10 | Liquid | Sediment | Hydrogenotrophic and/or aceticlastic methanogens | H2, acetate | CO2 | CO2 or acetate | NH4+ | 10 | 7.2 | Cysteine (0.05%) |
| SPG-11 | Liquid | Sediment | Methanogens | Methanol | CO2 | CO2 or methanol | NH4+ | 10 | 7.2 | Cysteine (0.05%), FeCl2 (1 mM) |
| SPG-12 | Liquid | Sediment | Heterotrophic sulfate reducers | Lactate, yeast extract | SO42– | Lactate, yeast extract | NH4+ | 10 | 7.2 | |
| SPG-13 | Liquid | Sediment | Sulfur reducers | H2, yeast extract | S2O32– | H2, yeast extract | NH4+ | 10 | 7.2 | |
| SPG-14 | Liquid | Sediment | Hydrogen and/or acetate-oxidizing sulfur reducers | H2, acetate | S2O32– | CO2, acetate | NH4+ | 10 | 7.2 | |
| SPG-15 | Liquid | Sediment | Fermenters | Casamino acids, yeast extract | Casamino acids, yeast extract | Casamino acids, yeast extract | NH4+ | 10 | 7.2 | |
| SPG-16 | Liquid | Sediment | Sulfate reducers | H2, acetate | SO42– | CO2 | NH4+ | 10 | 7.2 | |
| SPG-17 | Liquid | Sediment | Nitrate reducers | H2, yeast extract | NO3– | CO2 | NH4+ | 10 | 7.2 | |
| SPG329-1 | Liquid | Sediment | Aerobic heterotrophs | Various dissolved organic matter | O2 | Various dissolved organic matter | Various dissolved organic matter | 10 | 7.0 | |
| SPG329-2 | Liquid | Sediment | Fermenters | Various dissolved organic matter | Various dissolved organic matter | Various dissolved organic matter | Various dissolved organic matter | 10 | 7.0 | |
| SPG329-3 | Liquid | Sediment | Autotrophic hydrogenotropic nitrate reducers | H2 | NO3– | CO2 | NO3– | 10 | 7.0 | |
| SPG329-4 | Liquid | Sediment | Heterotrophic nitrate reducers | Various dissolved organic matter | NO3– | Various dissolved organic matter | Various dissolved organic matter | 10 | 7.0 | |
| SPG329-5 | Liquid | Sediment | Aerobic ammonium-oxidizing autotrophs | NH4+ | O2 | CO2 | NH4+ | 10 | 7.0 | |
| SPG329-6 | Liquid | Sediment | Aerobic autotrophic nitrite oxidizers | NO2– | O2 | CO2 | NO2– | 10 | 7.0 | |
| SPG329-7 | Liquid | Sediment | Anaerobic hydrogenotrophs | H2 | CO2 | CO2, acetate, methylated compounds | NH4+ | 10 | 7.0 | |
| SPG329-8 | Liquid | Sediment | Aerobic hydrogenotrophic autotrophs | H2 | O2 | CO2 | NH4+ | 10 | 7.0 | |
| SPG329-9 | Liquid | Sediment | Autotrophic iron-oxidizing autotrophs | Fe2+ | O2 | CO2 | NH4+ | 10 | 7.0 | |
| Marine 2216 (Difco) | Solid and liquid | Sediment | Aerobic heterotrophs | Various dissolved organic matter | O2 | Various dissolved organic matter | Various dissolved organic matter | 10 | ~7.6 | 1%, 10%, and 100% of media |
| Marine R2A (Fluka) | Solid | Sediment | Aerobic heterotrophs | Various dissolved organic matter | O2 | Various dissolved organic matter | Various dissolved organic matter | 10 | ~7.2 | Prepared with seawater |
| TSA agar (Oxoid) | Solid | Sediment | Aerobic heterotrophs | Various dissolved organic matter | O2 | Various dissolved organic matter | Various dissolved organic matter | 10 | ~7.3 | Prepared with seawater |
| TSB (Oxoid) | Liquid | Sediment | Aerobic heterotrophs | Various dissolved organic matter | O2 | Various dissolved organic matter | Various dissolved organic matter | 10 | ~7.3 | Prepared with seawater |
| TCBS agar (Difco) | Solid | Sediment | Vibrios | Various dissolved organic matter | O2 | Various dissolved organic matter | Various dissolved organic matter | 10 | ~8.6 | |
| Actinobacteria agar | Solid | Sediment | Actinobacteria | Various dissolved organic matter | O2 | Various dissolved organic matter | Various dissolved organic matter | 10 | ~7.6 | Prepared with seawater |
| Sediment extract agar | Solid | Sediment | Aerobic bacteria | Various dissolved organic matter | O2 | Various dissolved organic matter | Various dissolved organic matter | 10 | ~7.6 | |
| Monomer mix medium-1 (Mmm1) | Liquid | Sediment | Aerobic heterotrophs | Various dissolved organic matter | O2 | Various dissolved organic matter | Various dissolved organic matter | 4 | ~7.8 | Setup for all sites + various depths + dilution series (no oxic incubations at SPG-12) |
| Monomer mix medium-2 (Mmm2) | Liquid | Sediment | Anaerobic heterotrophs | Various dissolved organic matter | Various (no O2) | Various dissolved organic matter | Various dissolved organic matter | 4 | ~7.8 | |
| SPG-ASW | Liquid | Sediment | Hydrogenotrophic autotrophs | H2 | Various | CO2 | NH4+ | 4 | ~7.8 | |
| SPG-JL | Liquid | Sediment and basalt | Anaerobic methane-oxidizing consortium | Various dissolved organic matter, H2 | NO3–, SO42– | Various dissolved organic matter | Various dissolved organic matter | 10 | ~7.8 | 0.01% marine broth 2216 is supplemented. Primarily targeting Site U1371 |
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