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Table T9. Radioactive and stable isotopic substrates used for incubation experiments of sediment slurry, Expedition 329.
|
Experiment type
|
Metabolism
|
Compound
|
Radioactive isotope
|
Stable isotope
|
|
Activity (kBq/µL)
|
Added volume
(µL)
|
Activity
in vial
(kBq/vial)
|
Specific
activity
(kBq/µM)
|
Concentration label
(µM/µL) (M)
|
Label
(%)
|
Added
volume (µL)
|
Final concentration in vial (µM)
|
|
A
|
Autotrophy
|
14C bicarbonate
|
3.70
|
80
|
296
|
1.42
|
|
|
|
|
|
B
|
Autotrophy
|
14C bicarbonate
|
3.70
|
80
|
296
|
1.42
|
|
|
|
|
|
Nitrification
|
15N ammonium
|
|
|
|
|
1
|
99
|
8
|
200
|
|
|
33P phosphate
|
37
|
8
|
296
|
3700
|
|
|
|
|
|
C
|
Autotrophy
|
14C bicarbonate
|
3.70
|
80
|
296
|
1.42
|
|
|
|
|
|
N fixation
|
15N dinitrogen gas
|
|
|
|
|
|
99
|
|
1 mL gas
|
|
|
33P phosphate
|
37
|
8
|
296
|
3700
|
|
|
|
|
|
D
|
Autotrophy
|
14C bicarbonate
|
3.70
|
80
|
296
|
1.42
|
|
|
|
|
|
Organic P uptake
|
33P ATP
|
37
|
8
|
296
|
1.11E+08
|
|
|
|
|
|
E
|
Heterotrophy
|
14C acetate
|
0.84
|
80
|
67
|
2146
|
|
|
|
|
|
F
|
Heterotrophy
|
14C acetate
|
0.84
|
80
|
67
|
2146
|
|
|
|
|
|
Nitrification
|
15N ammonium
|
|
8
|
|
|
1
|
99
|
8
|
200
|
|
|
33P phosphate
|
37
|
8
|
296
|
3700
|
|
|
|
|
|
G
|
Heterotrophy
|
14C acetate
|
0.84
|
80
|
67
|
2146
|
|
|
|
|
|
N fixation
|
15N dinitrogen gas
|
|
|
|
|
|
99
|
|
1 mL gas
|
|
|
33P phosphate
|
37
|
8
|
296
|
3700
|
|
|
|
|
|
H
|
Nitrate reduction
|
15N nitrate
|
|
8
|
|
|
1
|
99
|
8
|
200
|
|
Autotrophy
|
13C bicarbonate
|
|
80
|
|
|
1
|
99
|
80
|
2000
|
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