Table T1. Cultivation media used in this study.
| Target microorganisms | Medium | Headspace gas | Reference |
|---|---|---|---|
| Methanogens | MMJ medium | H2:CO2 (80:20, 300 kPa) | Takai et al., 2002 |
| Anaerobic hydrogen oxidizers |
DSMZ medium 383 using the basic seawater medium with resazurin (1 mg/L) instead of Solution A |
H2:N2:CO2 (70:20:10, 200 kPa) | www.dsmz.de/microorganisms/medium/ pdf/DSMZ_Medium383.pdf |
| Aerobic hydrogen oxidizers |
Basic seawater medium supplemented with the following per 1 L: 0.5 g NaHCO3, 2.5 mg NiCl2·6H2O, 2.5 mg Na2WO4·2H2O, 0.1 mg Na2SeO3·5H2O, 1 mL trace element solution SL-10 (DSMZ medium 320), and 1 mL vitamin complex solution (DSMZ medium 141) |
H2:N2:O2:CO2 (49:47:3:1, 200 kPa) | www.dsmz.de/microorganisms/medium/ pdf/DSMZ_Medium320.pdf www.dsmz.de/microorganisms/medium/ pdf/DSMZ_Medium141.pdf |
| Aerobic sulfur oxidizers |
The medium described above for aerobic hydrogen oxidizers with 1 mM sodium thiosulfate and ~0.1 g elemental sulfur per tube |
N2:O2 (95:5, 100 kPa) |
Basic seawater medium = 0.06 g KH2PO4, 0.17 g Na2HPO4, and 0.25 g NH4NO3 per L of artificial seawater MARINE ART SF-1. MMJ = marine medium salts in artificial seawater.
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