Table T2. Detection of anaerobic microorganisms in the enrichment cultures from the basaltic rock cores.
Target metabolism: | Methanogen | Hydrogen oxidizer (sulfate reducer and nitrate reducer) | ||||||
---|---|---|---|---|---|---|---|---|
Energy source: | Hydrogen | Hydrogen | ||||||
Temperature: | 15°C | 37°C | 55°C | 15°C | 37°C | |||
Examination by: | GC | GC | GC | PCR | PCR | 16S rRNA gene sequences* | ||
Core | Depth (mbsf) | Section, piece | ||||||
336-U1382A- | ||||||||
3R | 113.6–118.3 | 1A | – | – | – | – | – | |
2B | – | – | – | – | – | |||
3A | – | – | – | – | + | Bacillus niacini 98% (Bacilli) | ||
4A | – | – | – | – | – | |||
4B | – | – | – | – | + | Methylobacterium fujisawaense 99% (AP) | ||
4R | 123.0–124.8 | 2E | – | – | – | – | – | |
5R | 132.5–134.6 | 1B | – | – | – | – | + | Bacillus niacini 98% (Bacilli) |
2E | – | – | – | – | – | |||
6R | 142.4–143.8 | 1A | – | – | – | – | + | Sphingomonas ursincola 99% (AP) |
1C | – | – | – | – | – | |||
7R | 153.1–153.9 | 2B | – | – | – | – | – | |
8R | 161.3–164.0 | 1C | – | – | – | – | – | |
4D | – | – | – | – | – | |||
4E | – | – | – | – | – | |||
9R | 171.5–173.8 | 1B | – | – | – | – | + | Not identified |
336-U1383C- | ||||||||
2R | 69.9–72.2 | 1C | – | – | – | – | + | Bacillus niabensis 99% (Bacilli) |
2D | – | – | – | – | – | |||
4R | 86.6–88.1 | 2D | – | – | – | – | – | |
5R | 96.4–97.2 | 1A | – | – | – | – | + | Bacillus niabensis 99% (Bacilli) |
8R | 124.7–126.0 | 1B | – | – | – | – | – | |
11R | 153.8–154.5 | 1C | – | – | – | – | – | |
17R | 202.1–203.1 | 1A | – | – | – | – | – | |
19R | 212.1–212.6 | 1A | – | – | – | – | + | Bacillus niacini 99% (Bacilli) |
1C | – | – | – | – | + | Bacillus niabensis 99% (Bacilli) | ||
20R | 219.4–220.7 | 1A | – | – | – | – | – | |
23R | 248.1–248.3 | 1A | – | – | – | – | – | |
24R | 256.6–257.1 | 1A | – | – | – | – | – | |
29R | 299.9–300.0 | 1A | – | – | – | – | – | |
30R | 304.0–306.3 | 1A | – | – | – | – | – | |
2B | – | – | – | – | – | |||
3C | – | – | – | – | – | |||
31R | 312.2–314.8 | 1B | – | – | – | – | – | |
336-U1384A- | ||||||||
12X | 95.2 | 1C | – | – | – | – | – |
* = closest microorganisms indicated by Blast search. GC = gas chromatography, PCR = polymerase chain reaction. Phylogenetic groups are shown in parentheses. AP = Alphaproteobacteria.
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