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Table T2. Detection of anaerobic microorganisms in the enrichment cultures from the basaltic rock cores.

Target metabolism: Methanogen Hydrogen oxidizer (sulfate reducer and nitrate reducer)
Energy source: Hydrogen Hydrogen
Temperature: 15°C 37°C 55°C 15°C 37°C
Examination by: GC GC GC PCR PCR 16S rRNA gene sequences*
Core Depth (mbsf) Section, piece            
336-U1382A-
3R    113.6–118.3     1A  
2B  
3A + Bacillus niacini 98% (Bacilli)
4A  
4B + Methylobacterium fujisawaense 99% (AP)
4R 123.0–124.8 2E  
5R 132.5–134.6 1B + Bacillus niacini 98% (Bacilli)
2E  
6R 142.4–143.8 1A + Sphingomonas ursincola 99% (AP)
1C  
7R 153.1–153.9 2B  
8R 161.3–164.0 1C  
4D  
4E  
9R 171.5–173.8 1B + Not identified
336-U1383C-
2R 69.9–72.2 1C + Bacillus niabensis 99% (Bacilli)
2D  
4R 86.6–88.1 2D  
5R 96.4–97.2 1A + Bacillus niabensis 99% (Bacilli)
8R 124.7–126.0 1B  
11R 153.8–154.5 1C  
17R 202.1–203.1 1A  
19R 212.1–212.6 1A + Bacillus niacini 99% (Bacilli)
1C + Bacillus niabensis 99% (Bacilli)
20R 219.4–220.7 1A  
23R 248.1–248.3 1A  
24R 256.6–257.1 1A  
29R 299.9–300.0 1A  
30R 304.0–306.3 1A  
2B  
3C  
31R 312.2–314.8 1B  
336-U1384A-
12X 95.2 1C  

* = closest microorganisms indicated by Blast search. GC = gas chromatography, PCR = polymerase chain reaction. Phylogenetic groups are shown in parentheses. AP = Alphaproteobacteria.

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