Table T15. Artificial salt solution used as the basis for all enrichment media, Expedition 317. (See table note.)
| Component | Concentration |
|---|---|
| Distilled water | 1000 mL |
| NaCl | 30 g/L |
| KBr | 0.09 g/L |
| CaCl2·2H2O | 1.47 g/L |
| MgCl2·6H2O | 5.67 g/L |
| KCl | 0.6 g/L |
| MgSO4·7H2O | 5.62 g/L |
| Tris | 1.20 g/L |
| SrCl2·6H2O | 0.02 g/L |
| The pH was adjusted to 7.5 with 1M HCl | |
| After autoclaving, the following were added from sterile stock solutions: | |
| NH4Cl (1M) | 4.67 mL/L |
| KH2PO4 (1M) | 1.5 mL/L |
| Trace element solution | 1.0 mL/L |
| Vitamin mixture | 1.0 mL/L |
Note: The trace element solution and vitamin mixture was described by Widdel et al. (1983).
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