Table T3. Detection of aerobic microorganisms in the enrichment cultures from the basaltic rock cores.
| Target metabolism: | Hydrogen oxidizer | Sulfur oxidizer | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Energy source: | Hydrogen | Elemental sulfur and thiosulfate | |||||||||
| Temperature: | 8°C | 25°C | 37°C | 15°C | 37°C | ||||||
| Examination by: | PCR | 16S rRNA gene sequences | PCR | 16S rRNA gene sequences | PCR | 16S rRNA gene sequences | PCR | PCR | 16S rRNA gene seq. | ||
| Core | Depth (mbsf) | Section, piece | |||||||||
| 336-U1382A- | |||||||||||
| 3R | 113.6–118.3 | 1A | – | – | – | – | – | ||||
| 2B | – | + | Ralstonia pickettii 99%–100% (BP) | – | – | – | |||||
| 3A | – | – | + | Paenibacillus darwinianus 97% (Bacilli) | – | – | |||||
| 4A | – | – | – | – | – | ||||||
| 4B | – | – | – | – | – | ||||||
| 4R | 123.0–124.8 | 2E | – | – | – | – | – | ||||
| 5R | 132.5–134.6 | 1B | + | Pseudomonas fluorescens 98% (GP) | – | – | – | – | |||
| 2E | – | – | – | – | – | ||||||
| 6R | 142.4–143.8 | 1A | + | Salinibacterium amurskyense 100% (Act) Sphingomonas changbaiensis 96% (AP) Sphingomonas indica 97% (AP) | + | Not identified | – | – | – | ||
| 1C | + | Ralstonia pickettii 99%–100% (BP) | – | + | Paenibacillus thermoaerophilus 92% (Bacilli) | – | – | ||||
| 7R | 153.1–153.9 | 2B | + | Not identified | – | – | – | + | Moraxella osloensis 98% (GP) | ||
| 8R | 161.3–164.0 | 1C | – | + | Pseudomonas tolaasii 97% (GP) | – | – | – | |||
| 4D | – | – | – | – | – | ||||||
| 4E | – | – | – | – | – | ||||||
| 9R | 171.5–173.8 | 1B | + | Pseudomonas fluorescens 98% (GP) | + | Ralstonia pickettii 99%–100% (BP) | – | – | – | ||
| 336-U1383C- | |||||||||||
| 2R | 69.9–72.2 | 1C | – | – | + | Paenibacillus thermoaerophilus 92% (Bacilli) | – | – | |||
| 2D | + | Ralstonia pickettii 99%–100% (BP) | – | + | Acidovorax ebreus 99% (BP) | – | + | Not identified | |||
| 4R | 86.6–88.1 | 2D | + | Pseudomonas fluorescens 98% (GP) | – | – | – | – | |||
| 5R | 96.4–97.2 | 1A | – | – | + | Acidovorax ebreus 99% (BP) | – | – | |||
| 8R | 124.7–126.0 | 1B | + | Ralstonia pickettii 99%–100% (BP) | – | + | Not identified | – | – | ||
| 11R | 153.8–154.5 | 1C | – | – | + | Not identified | – | – | |||
| 17R | 202.1–203.1 | 1A | + | Sphingomonas paucimobilis 99% (AP) | – | + | Phenylobacterium koreense 94% (AP) | – | – | ||
| 19R | 212.1–212.6 | 1A | – | – | + | Acidovorax ebreus 99% (BP) | – | – | |||
| 1C | – | – | + | Paenibacillus darwinianus 97% (Bacilli) | – | – | |||||
| 20R | 219.4–220.7 | 1A | – | – | – | – | – | ||||
| 23R | 248.1–248.3 | 1A | – | – | – | – | – | ||||
| 24R | 256.6–257.1 | 1A | – | Exophiala sp. (Fungi) | + | Paenibacillus darwinianus 97% (Bacilli) | – | – | |||
| 29R | 299.9–300.0 | 1A | + | Ralstonia pickettii 99%–100% (BP) | + | Not identified | + | Paenibacillus thermoaerophilus 92% (Bacilli) | – | – | |
| 30R | 304.0–306.3 | 1A | – | + | Pseudomonas fluorescens 99% (GP) | – | – | – | |||
| 2B | – | + | Ralstonia pickettii 99%–100% (BP) | – | – | – | |||||
| 3C | – | – | + | Acidovorax ebreus 99% (BP) | – | – | |||||
| 31R | 312.2–314.8 | 1B | – | – | – | – | – | ||||
| 336-U1384A- | |||||||||||
| 12X | 95.2 | 1C | – | – | – | – | – | ||||
PCR = polymerase chain reaction. 16S rRNA gene sequences = closest microorganisms indicated by Blast search. Phylogenetic groups are shown in parentheses. AP = Alphaproteobacteria, BP = Betaproteobacteria, GP = Gammaproteobacteria, Act = Actinobacteria. Not identified = direct sequencing did not succeed.
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