Table T4. Detection of microorganisms in the sediment cores: real-time PCR for methanogens and the cultivation of sulfur oxidizers
| Target metabolism: | Methanogen (anaerobic) | Sulfur oxidizer (aerobic) | ||||
|---|---|---|---|---|---|---|
| Energy source: | — | Elemental sulfur and thiosulfate | ||||
| Incubation temperature: | — | 15°C | 37°C | |||
| Examination by: | Q-PCR | PCR | 16S rRNA gene sequences | PCR | ||
| Core | Section | Depth (mbsf) | ||||
| 336-U1382B- | ||||||
| 1H | 4 | 3.90–4.00 | – | – | – | |
| 2H | 6 | 12.87–12.97 | – | – | – | |
| 3H | 5 | 21.90–22.00 | – | – | – | |
| 4H | 4 | 28.65–28.75 | – | – | – | |
| 6H | 7 | 50.28–50.38 | – | – | – | |
| 7H | 6 | 60.61–60.71 | – | + | Paracoccus marcusii 100% (AP) | – |
| 8H | 6 | 69.95–70.05 | – | – | – | |
| 10H | 5 | 86.91–87.01 | – | – | – | |
| 336-U1383D- | ||||||
| 1H | 3 | 2.54–2.64 | – | – | – | |
| 2H | 5 | 11.25–11.35 | – | – | – | |
| 3H | 6 | 22.27–22.37 | – | – | – | |
| 4H | 5 | 30.08–30.18 | – | – | – | |
| 5H | 5 | 39.80–39.90 | – | + | Pseudomonas xanthomarina 99% (GP) | – |
| 336-U1383E- | ||||||
| 1H | 2 | 2.0–2.1 | – | + | Halomonas axialensis 99% (GP) | – |
| 2H | 6 | 10.58–10.68 | – | – | – | |
| 3H | 6 | 20.08–20.18 | – | + | Halomonas axialensis 98% (GP) | – |
| 4H | 5 | 27.06–27.16 | – | + | Pseudomonas xanthomarina 99% (GP) | – |
| 5H | 4 | 36.25–36.35 | – | – | – | |
| 6H | 5 | 45.7–47.0* | – | + | Marinobacter adhaerens 98% (GP) | – |
| 336-U1384A- | ||||||
| 1H | 2 | 1.97–2.07 | – | + | Marinobacter adhaerens 99% (GP) | – |
| 2H | 6 | 12.04–12.14 | – | – | – | |
| 3H | 5 | 19.78–19.88 | – | – | – | |
| 4H | 6 | 30.79–30.89 | – | – | – | |
| 5H | 6 | 40.04–40.14 | – | – | – | |
| 6H | 4 | 47.90–48.00 | – | – | – | |
| 7H | 4 | 57.08–57.18 | – | – | – | |
| 8H | 5 | 67.47–67.57 | – | – | – | |
| 9H | 5 | 76.91–77.01 | – | – | – | |
| 10H | 5 | 86.5–86.6 | – | – | – | |
| 11H | 2 | 91.3–91.4 | – | – | – | |
* = depth at which the master core for microbiology was collected. Q-PCR = real-time PCR experiment. PCR = polymerase chain reaction. 16S rRNA gene sequences = closest microorganisms indicated by Blast search. Phylogenetic groups are shown in parentheses. AP = Alphaproteobacteria, GP = Gammaproteobacteria.
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