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Table T15. Artificial salt solution used as the basis for all enrichment media, Expedition 317. (See table note.)

Component Concentration
Distilled water 1000 mL
NaCl 30 g/L
KBr 0.09 g/L
CaCl2·2H2O 1.47 g/L
MgCl2·6H2O 5.67 g/L
KCl 0.6 g/L
MgSO4·7H2O 5.62 g/L
Tris 1.20 g/L
SrCl2·6H2O 0.02 g/L
The pH was adjusted to 7.5 with 1M HCl
After autoclaving, the following were added from sterile stock solutions:
NH4Cl (1M) 4.67 mL/L
KH2PO4 (1M) 1.5 mL/L
Trace element solution 1.0 mL/L
Vitamin mixture 1.0 mL/L

Note: The trace element solution and vitamin mixture was described by Widdel et al. (1983).

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