Proc. IODP, 336, Summary of carbon, nitrogen, and iron leaching characteristics and fluorescence properties of materials considered for subseafloor observatory assembly

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Chapter contents Abstract Introduction Materials and methods Construction of Expedition 336 CORK observatories Leaching experiments Fluorescence tests with pipe dopes and sealants Results Visible changes in materials during incubation Carbon leaching from borehole materials Nitrogen leaching from borehole materials Fluorescence characteristics of dopes and sealants Discussion Acknowledgments References Figures F1. Coatings and sealants. F2. Materials tested for leaching. F3. Amerlock- and Xlyan-coated steel coupons. F4. Fluorescence of sealants and pipe dope. Tables T1. Carbon leaching rates. T2. Nitrogen leaching rates. PDF file			Previous \| Next doi:10.2204/iodp.proc.336.108.2012 Summary of carbon, nitrogen, and iron leaching characteristics and fluorescence properties of materials considered for subseafloor observatory assembly¹ Beth N. Orcutt,^2,³ Roman A. Barco,³ Samantha B. Joye,⁴ and Katrina J. Edwards^2,⁵ Abstract To limit the potential for metal contamination in crustal subsurface borehole observatories, fiberglass and resin-coated steel pipe were used for the first time in scientific ocean drilling during Integrated Ocean Drilling Program (IODP) Expeditions 327 and 336. Here, we summarize the carbon, nitrogen, and iron leaching characteristics of the various materials used and considered for the observatory construction, as these substrates may potentially induce or inhibit in situ microbial growth. We also report on the fluorescence properties of several dopes and sealants used in observatory construction, as these may interfere with DNA-based fluorescence observations on observatory microbial colonization experiments. ¹Orcutt, B.N., Barco, R.A., Joye, S.B., and Edwards, K.J., 2012. Summary of carbon, nitrogen, and iron leaching characteristics and fluorescence properties of materials considered for subseafloor observatory assembly. In Edwards, K.J., Bach, W., Klaus, A., and the Expedition 336 Scientists, Proc. IODP, 336: Tokyo (Integrated Ocean Drilling Program Management International, Inc.). doi:10.2204/iodp.proc.336.108.2012 ²Expedition 336 Scientists’ addresses. ³University of Southern California, 3616 Trousdale Parkway, Los Angeles CA 90089, USA. ⁴Department of Marine Sciences, University of Georgia, Athens GA 30602-3636, USA. ⁵Correspondence author: kje@usc.edu Publication: 16 November 2012 MS 336-108 Top of page \| Previous \| Next