Proc. IODP, 336, Data report: total cell counts and qPCR abundance of Archaea and Bacteria in shallow subsurface marine sediments of North Pond: gravity cores collected during site survey cruise prior to IODP Expedition 336

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Chapter contents Introduction Materials and methods Results and conclusions Acknowledgments References Figures F1. qPCR analysis of sediments. F2. Scanning electron microscopy images. F3. Total cell counts and qPCR abundance. PDF file			Previous \| Next doi:10.2204/iodp.proc.336.201.2013 References D’Hondt, S., Jørgensen, B.B., Miller, D.J., Batzke, A., Blake, R., Cragg, B.A., Cypionka, H., Dickens, G.R., Ferdelman, T., Hinrichs, K.-U., Holm, N.G., Mitterer, R., Spivack, A., Wang, G., Bekins, B., Engelen, B., Ford, K., Gettemy, G., Rutherford, S.D., Sass, H., Skilbeck, C.G., Aiello, I.W., Guerin, G., House, C.H., Inagaki, F., Meister, P., Naehr, T., Niitsuma, S., Parkes, R.J., Schippers, A., Smith, D.C., Teske, A., Wiegel, J., Naranjo Padillo, C., and Solis Acosta, J.L., 2004. Distributions of microbial activities in deep subseafloor sediments. Science, 306(5705):2216–2221. doi:10.1126/science.1101155 Durbin, A.M., and Teske, A., 2012. 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Microbiology, 148:257–266. http://mic.sgmjournals.org/content/148/1/257.full.pdf Parkes, R.J., Cragg, B.A., Bale, S.J., Getliff, J.M., Goodman, K., Rochelle, P.A., Fry, J.C., Weightman, A.J., and Harvey, S.M., 1994. Deep bacterial biosphere in Pacific Ocean sediments. Nature (London, U. K.), 371(6496):410–413. doi:10.1038/371410a0 Røy, H., Kallmeyer, J., Adhikari, R.R., Pockalny, R., Jørgensen, B.B., and D’Hondt, S., 2012. Aerobic microbial respiration in 86-million-year-old deep-sea red clay. Science, 336(6083):922–925. doi:10.1126/science.1219424 Schippers, A., Kock, D., Höft, C., Köweker, G., and Siegert, M., 2012. Quantification of microbial communities in subsurface marine sediments of the Black Sea and off Namibia. Front. Extreme Microbiol., 3:16. doi:10.3389/fmicb.2012.00016 Schippers, A., Neretin, L.N., Kallmeyer, J., Ferdelman, T.G., Cragg, B.A., Parkes R.J., and Jørgensen, B.B., 2005. Prokaryotic cells of the deep sub-seafloor biosphere identified as living bacteria. Nature (London, U. K.), 433(7028):861–864. doi:10.1038/nature03302 Takai, K., and Horikoshi, K., 2000. Rapid detection and quantification of members of the archaeal community by quantitative PCR using fluorogenic probes. Appl. Environ. Microbiol., 66(11):5066–5072. doi:10.1128/AEM.66.11.5066-5072.2000 Valentine, D.L., 2007. Adaptations to energy stress dictate the ecology and evolution of the archaea. Nat. Rev. Microbiol., 5(4):316–323. doi:10.1038/nrmicro1619 Webster, G., Newberry, C.J., Fry, J.C., and Weightman, A.J., 2003. Assessment of bacterial community structure in the deep sub-seafloor biosphere by 16S rDNA-based techniques: a cautionary tale. J. Microbiol. Methods, 55(1):155–164. doi:10.1016/S0167-7012(03)00140-4 Weinbauer, M.G., Beckmann, C., and Höfle, M.G., 1998. Utility of green fluorescent nucleic acid dyes and aluminium oxide membrane filters for rapid epifluorescence enumeration of soil and sediment bacteria. Appl. Environ. 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