Proc. IODP, 329, Preliminary experiment for cell count using flow cytometry

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Chapter contents Abstract Introduction Principles of fluorescent wavelength–based cell discrimination Standardization of flow cytometry cell count protocol for South Pacific Gyre sediment Protocol FCM-A (basic protocol prior to modifications) Protocol FCM-B (protocol without CaF₂ precipitation) Protocol FCM-C (protocol without HF treatment) Protocol FCM-D (final improved protocol with HF treatment) Quality assurance and control and flow cytometry cell count data processing Results and discussion Flow cytometry quality control Conclusion and future prospects Acknowledgments References Figures F1. Sediment cell counts. F2. Control experiments. PDF file			Previous \| Next doi:10.2204/iodp.proc.329.110.2011 Acknowledgments This research used samples and data provided by the Integrated Ocean Drilling Program (IODP) and is supported in part by the JSPS Strategic Fund for Strengthening Leading-Edge Research and Development (to JAMSTEC). Top of page \| Previous \| Next

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