Proc. IODP, 329, Preliminary experiment for cell count using flow cytometry

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Chapter contents Abstract Introduction Principles of fluorescent wavelength–based cell discrimination Standardization of flow cytometry cell count protocol for South Pacific Gyre sediment Protocol FCM-A (basic protocol prior to modifications) Protocol FCM-B (protocol without CaF₂ precipitation) Protocol FCM-C (protocol without HF treatment) Protocol FCM-D (final improved protocol with HF treatment) Quality assurance and control and flow cytometry cell count data processing Results and discussion Flow cytometry quality control Conclusion and future prospects Acknowledgments References Figures F1. Sediment cell counts. F2. Control experiments. PDF file			Previous \| Next doi:10.2204/iodp.proc.329.110.2011 References D’Hondt, S., Spivack, A.J., Pockalny, R., Ferdelman, T.G., Fischer, J.P., Kallmeyer, J., Abrams, L.J., Smith, D.C., Graham, D., Hasiuk, F., Schrum, H., and Stancin, A.M., 2009. Subseafloor sedimentary life in the South Pacific Gyre. Proc. Nat. Acad. Sci., U. S. A., 106(28):11651–11656. doi:10.1073/pnas.0811793106 Expedition 329 Scientists, 2011a. Methods. In D’Hondt, S., Inagaki, F., Alvarez Zarikian, C.A., and the Expedition 329 Scientists, Proc. IODP, 329: Tokyo (Integrated Ocean Drilling Program Management International, Inc.). doi:10.2204/iodp.proc.329.102.2011 Expedition 329 Scientists, 2011b. Site U1365. In D’Hondt, S., Inagaki, F., Alvarez Zarikian, C.A., and the Expedition 329 Scientists, Proc. IODP, 329: Tokyo (Integrated Ocean Drilling Program Management International, Inc.). doi:10.2204/iodp.proc.329.103.2011 Expedition 329 Scientists, 2011c. Site U1368. In D’Hondt, S., Inagaki, F., Alvarez Zarikian, C.A., and the Expedition 329 Scientists, Proc. IODP, 329: Tokyo (Integrated Ocean Drilling Program Management International, Inc.). doi:10.2204/iodp.proc.329.106.2011 Kallmeyer, J., Smith, D.C., Spivack, A.J., and D’Hondt, S., 2008. New cell extraction procedure applied to deep subsurface sediments. Limnol. Oceanogr.: Methods, 6:236–245. http://www.aslo.org/lomethods/free/2008/0236.pdf Morono, Y., Terada, T., Masui, N., and Inagaki, F., 2009. Discriminative detection and enumeration of microbial life in marine subsurface sediments. ISME J., 3(5):503–511. doi:10.1038/ismej.2009.1 Morono, Y., and Inagaki, F., 2010. Automatic slide-loader fluorescent microscope for discriminative enumeration of subseafloor life. Sci. Drill., 9:32–36. doi:10.2204/iodp.sd.9.06.2010 Top of page \| Previous \| Next

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