Proc. IODP, 336, Data report: cultivation of microorganisms from basaltic rock and sediment cores from the North Pond on the western flank of the Mid-Atlantic Ridge, IODP Expedition 336

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Chapter contents Abstract Introduction Methods and materials Subsampling of cores Preparation of growth media Enrichment cultures Bacterial and archaeal rRNA gene analyses Analysis of a fungal isolate Measurement of methane production Real-time PCR of the methyl coenzyme-M reductase subunit A gene (mcrA) Results Anaerobic enrichments from rock cores A fungal isolate from the rock core Acknowledgments References Figure F1. Position of the isolate NPf1 among representative species of the order Chaetothyriales. Tables T1. Cultivation media. T2. Anaerobic microorganisms. T3. Aerobic microorganisms. T4. Microorganisms. PDF file			Previous \| Next doi:10.2204/iodp.proc.336.204.2015 Data report: cultivation of microorganisms from basaltic rock and sediment cores from the North Pond on the western flank of the Mid-Atlantic Ridge, IODP Expedition 336¹ Hisako Hirayama,² Mariko Abe,² Junichi Miyazaki,² Sanae Sakai,² Yuriko Nagano,³ and Ken Takai² Abstract Cultivation experiments targeting chemolithoautotrophic microorganisms were performed using subseafloor basaltic cores (the deepest sample is from 315 meters below seafloor [mbsf] and overlying sediment cores (the deepest sample is from 91.4 mbsf) from North Pond on the western flank of the Mid-Atlantic Ridge. The cores were recovered by the R/V JOIDES Resolution during Integrated Ocean Drilling Program Expedition 336. Different bacteria were grown under different media and temperature conditions. In the enrichment cultures of the basaltic cores under aerobic conditions, frequently detected bacteria at 8°C and 25°C were members of the genera Ralstonia (the class Betaproteobacteria) and Pseudomonas (Gammaproteobacteria), whereas members of the genera Paenibacillus (Bacilli) and Acidovorax (Betaproteobacteria) were conspicuous at 37°C. Bacillus spp. (Bacilli) were outstanding at 37°C under anaerobic conditions. In the enriched cultures of the sediment cores, bacterial growth was observed at 15°C but not at 37°C, and the bacteria detected at 15°C mostly belonged to gammaproteobacterial genera such as Pseudomonas, Halomonas, and Marinobacter. All of the bacteria detected in this study were enriched only, and subcultivation of the enriched cultures in the respective original media did not succeed. The presence of hydrogenotrophic methanogens was examined by a culture-dependent or a culture-independent analysis in the basalt and sediment cores but was not proven. A fungal isolate was obtained from a single basaltic core and belonged to the genus Exophiala of the order Chaetothyriales. ¹ Hirayama, H., Abe, M., Miyazaki, J., Sakai, S., Nagano, Y., and Takai, K., 2015. Data report: cultivation of microorganisms from basaltic rock and sediment cores from the North Pond on the western flank of the Mid-Atlantic Ridge, IODP Expedition 336. In Edwards, K.J., Bach, W., Klaus, A., and the Expedition 336 Scientists, Proc. IODP, 336: Tokyo (Integrated Ocean Drilling Program Management International, Inc.). doi:10.2204/iodp.proc.336.204.2015 ² Department of Subsurface Geobiological Analysis and Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan. Correspondence author: hirayamah@jamstec.go.jp ³ Department of Marine Biodiversity Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan. Initial receipt: 5 September 2014 Acceptance: 25 February 2015 Publication: 28 April 2015 MS 336-204 Top of page \| Previous \| Next