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doi:10.2204/iodp.proc.327.103.2011 MicrobiologyMicrobiological hard rock samples were collected from almost every RCB-cored interval of Hole U1362A, as well as from one bit sample from the sediment/basalt interface cored in Hole U1362B. Roughly 5% of the hard rock core that was recovered from Hole U1362A was taken as whole-round samples from the core splitting room and dedicated to microbiological analysis. The 25 hard rock microbiology samples span a range of lithologic units, alteration states, and chilled margin presences, and most contain at least one vein or fracture. Photographs of the samples are available in MICROBIO in “Supplementary material.” Shipboard scientists attempted to prepare total cell counts of rock samples fixed in formaldehyde solution and stained with either acridine orange or SYBR Green I DNA stains; however, a lack of antibleaching immersion oil during the expedition prevented stable cell-fluorescence signals and precluded accurate cell-count measurements. Microspheres were used during all coring operations to help in evaluating core contamination. Microsphere density was evaluated in all samples, generally both before and after the outer surfaces of rocks were flame sterilized prior to being split. Contamination checks (Table T8) indicate that microsphere density tends to be high in exterior alteration crusts and within glassy margins, whereas interior rock sections generally have no or low microsphere densities. Additionally, a few recovered plastic bags that held the fluorescent microsphere solutions in the core catcher were collected for contamination checks during shore-based DNA analysis. |
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