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doi:10.2204/iodp.proc.322.103.2010

Microbiology

Twelve 10 cm long whole-round cores were taken from Hole C0011B for molecular microbiology (phylogenetic) studies and stored at –80°C, including two for community archival purposes. Where dedicated microbiology whole-round sampling was not possible, twenty-six 10 cm³ "chips" were obtained from cleaned whole rounds used for interstitial water analyses.

In addition, ten 5 cm long whole-round cores were taken, subsampled for fluorescence in situ hybridization (FISH) and cell counting studies, and stored anaerobically at 4°C for shore-based cultivation studies. Eleven 2–3 cm³ samples were also taken from the 5 cm long whole-round cores. One of these samples came from the 10 cm long whole-round cores described above (prior to ultralow freezing). These samples were preserved for shore-based cell counting and FISH studies.

Three working-half split cores were sampled for roughly 10 cm³ carbonaceous mud fragments ("chunks"); these samples were obtained 2–3 h after X-ray CT scanning.

Forty-three 1–2 mL interstitial water samples were obtained for shipboard spectrophotometric analyses of ferrous iron and acid volatile sulfide. Data from these measurements are presented in "Inorganic geochemistry."

Table T25 presents the depth and type distribution of the samples obtained from Hole C0011B.

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